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Basa fish (Pangasianodon hypopthalmus) is the top fifth most consumed fish in Thailand. The production of Basa
fish generates a large number of by-products. Nowadays, most of these residues are recovered as animal feedstocks or fertilizer. However, it is interesting to explore another choice of waste management practice such as the recovery of valuable material. This study aimed to extract collagen from the skin and bone of Basa fish (Pangasianodon hypopthalmus) by using a green solvent. Two extraction methods were carried out. First, the conventional extraction was conducted by using acetic acid and compared with the physical extraction which employed water acidified with carbon dioxide. Conventional extraction was conducted using 0.75 M acetic acid at 4oC and solid-liquid ratio 1:40 (w/v). The physical extraction using water acidified by
carbon dioxide was conducted at 37oC, 10 bars and solid-liquid ratio 1:40 (w/v). The extraction time of each technique was varied from 3 to 24 hours. The FTIR results confirmed the
presence of amide A, amide B, amide I, amide II, and amide III of the extracted collagen obtained from these two techniques. When using fish skin and bone as raw material, the physical extraction technique increased the yield of dry weight collagen by 8 times and 7 times, respectively. Additionally, the physical extraction could shorten the processing time (from pretreatment until freeze-drying) by 56 hours.
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