Main Article Content
Diarrhea is a serious problem in developing countries. Shigella flexneri is the commonest cause bacillary diarrhea in developing countries. Vaccination against bacteria Shigella spp. necessary to reduce the incidence of diarrhea bacillary including those caused by S. flexneri. This study aims to explore the hemagglutinin protein (a protein that is able to agglutinate erythrocytes) pili and Omp S. flexneri alleged role in the adhesion of bacteria to the enterocytes. This study using hemagglutination assay method by reacting antigen derived either from protein sub units pili or subunits Omp S. flexneri result of purification, then observed agglutination occurs. At the protein sub units of S. flexneri pili showed: the molecular weight (MW) 72 kDa protein occurs in the agglutination titer 1/4, on the MW 27 kDa agglutination titer 1/16, and the MW 18 kDa occurred agglutination in titers of 1/128. While the Omp protein 72 kDa subunit; 27 kDa; and 18 kDa occurred agglutination in titers of 1/128. These results prove that the pili and Omp protein purification results thought to contain adhesion protein that is able to bind erythrocytes or called hemagglutinin protein. The occurrence of agglutination indicated by the absence of erythrocyte sediment at the bottom of the wells. While the deposition that occurs on the bottom of the well indicates a negative result, this is because the erythrocytes are not bound by a protein sub unit of pili and Omp S. flexneri.
Anam K., Nurdiana N., Hernowati T.E., Hidayat S. and Sumarno R.P. 2015. Cross Immunity among the 49.8 kDa Pili sub-unit Hemagglutinin Proteins and 7.9 kDa Pili sub-unit Anti Hemagglutinin Proteins of Shigella spp. Pak. J. Pharm. Sci., 2015 (accepted)
Anonymous, 2005. Shigellosis. Jakarta: Direktorat Jenderal PPPL Kementerian Kesehatan Republik Indonesia.
Burrows L. 2005. Weapons of mass retraction. Mol Microbial. Vol.57. p. 878-888
Chakrabarti M. A. 2010. Simple Approach Towards The Development Of A ShigellaVaccine. Al Ameen J Med Sci. 4: Vol. 3: 263-264.
Chmiela M, Lawnik M, Czkwianianc E, Rechciński T, Płaneta-Małecka I, Wadström T, Rudnicka W. 1997. Attachment of Helicobacter pylori strains to human epithelial cells. J Physiol Pharmacol. Sep:48(3):393-404.
Forest C. 2007. Contribution of the Fimbrial Operon of Salmonella enteric.SerovarTyphi during Interaction with Human Cells. Infect. Immun. 11 :Vol. 75. - hal. 5264-5271.
G-Biosciences. 2010. A Handbook & Selection Guide to Detergent and Detergent Removal [Online].www.GBiosciences.com. G-Biosciences
Hanne LF, Findkelstein RA.1982. Characterization and Distribution of the Hemagglutinin Produced by Vibriocholerae. Infect. Immun. 36:209-214
Hay. 2002. Practical Immunology. Blackwell Science
Herwana, 2010. Shigella-associated Diarrhea in Children in South Jakarta Indonesia. Southeast Asian J Trop Med Public Health, 41(2), pp. 418-25.
Laemli, U.K., 1970. Cleavage of Structural Protein during the Assembly of the Head of Bacteriophage T4, Nature. 227: 680-686.
Niyogi. 2005. Shigellosis. J Microbiol. The Journal of Microbiology. 2 : Vol.43 : 133-143.
Pore D and Chakrabarti M. 2013. Outer membrane protein A (OmpA) from Shigella flexneri 2a: A promising subunit vaccine candidate. Vaccine. 236 : Vol. 31 : 3644–3650
Pore D, Mahata N, Pal A, Chakrabarti MK. 2010. 34 kDa MOmp of Shigellaflexneri promotes TLR2 mediated macrophage activation with the engagement of NF-kappaB and p38 MAP kinase signaling. MolImmunol. May;47(9):1739-46
Proft, T and Baker, N. 2009. Pili in Gram negative and Gram positive bacteria - Structure, assembly and their Role in Disease. (review). Cell.Mol. Life Sci. 66. p. 613-635
Salyers and Whitt, 2002. Bacterial Pathogenesis: a Molecular Approach. 2 ed. Washington DC: American Society for Microbiology Press.
Schroeder, G. & Hilbi, H., 2008. Molecular Pathogenesis of Shigella spp : controlling Host Cell Signaling, Invasion, and Death by Type II Secretion.CLINICAL MICROBIOLOGY REVIEWS, 21(1), pp. 134-156.
Soto, G.E. and Hultgren, S.J. 1999. Minireview Bacterial adhesins: Common themes and variatoin inarchitecture and assembly. Infect. Immun. 181: 1059-1071
Starks, A.M.,Froenlich, B.J,. Jones, T.N., and Scott, J.R. 2006.Asembly of CsiPili: The Role of Specific Residues of the Mayor pilin, CooA. J. Bacteriol. 188(1): 231-239
Sumarno R.P., Uun Y., Winarsih, Santoso S., Samsul I. Detection of molecule adhesion subunit pili 48 kDaSalmonella typhi by immunochemistry method using sera patients suffering from typhoid fever. J. Basic. Appl. Sci. Res. 2-2012 : 8527-32.