Profile of Protein Levels Some Tobacco Varieties (Nicotiana tabacum L.) On Waterlogging Stress

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Tutik Nurhidayati Novitasari Novitasari Herry Purnobasuki Sucipto Hariyanto Nurul Jadid


Tobacco is a high-value crops that are sensitive to waterlogging stress. Some tobacco varieties have been widely cultivated in Indonesia, including Jepon Mawar, Jepon Banyak and Rejeb. Some species have different abilities to withstand the conditions of waterlogging stress by morphological adaptation, anatomy, physiology, and metabolic pathway changes. Changes in protein profiles is one form of plant defense response to waterlogging stress. Profile proteins experienced upregulation in hypoxia and anoxia conditions when gripped waterlogging stress known as anaerobic polypeptides. In addition to the waterlogging stress will cause the decrease in chlorophyll levels as a result of chlorosis during the stress. The purpose of this research is to know protein profile and chlorophyll content of several varieties of tobacco (Nicotiana tabacum L.): Rejeb, Jepon Mawar and Jepon Many against stagnant waterlogging stress. The protein profile was analyzed by SDS-PAGE method. While the chlorophyll content was analyzed by spectrophotometric method. The protein profiles expressed from the three test varieties were present in the molecular weight range 85.38-153.33 kDa. Proteins with a molecular weight of 85.38 kDa are thought to have similarities to the Peroxsidase group (BM = 85 kDa) that play a role in the ROS detoxification process. While the chlorophyll content in the three varieties decreased with the increasing of waterlogging stress except on Rejeb varieties treated by 175% and Jepon Mawar varieties on 200% waterlogging stress treatment.

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NURHIDAYATI, Tutik et al. Profile of Protein Levels Some Tobacco Varieties (Nicotiana tabacum L.) On Waterlogging Stress. Proceedings of the International Conference on Green Technology, [S.l.], v. 8, n. 1, p. 180-186, nov. 2017. ISSN 2580-7099. Available at: <>. Date accessed: 02 feb. 2023. doi:


[1] Sairam, R.K., Kumutha, D., Ezhilmathi, K., Deshmukh P.S, dan Srivastava, G.C. “Physiology and biochemistry of waterlogging tolerance in plants”. Journal Bio Plant (2008) 52:401–412.
[2] Barrett-Lennard, E.G. “The interaction between waterlogging and salinity in higher plants causes consequences and implications”. Journal Plant and Soil (2003) 253:35-44.
[3] Shimmamura, S., Yoshida, S., dan Mochizuki, T. “Cortical aerenchyma formation in hypocotil and advemtitious roots of Luffa cylindrical subjected to soil flooding”. Journal of Annals Botany (2007) 100:1431–1439.
[4] Armstrong, J. dan Armstrong, W. “Rice: sulphide–induced barriers to root radial oxygen loss, Fe2+ and water uptake, and lateral root emergence”. Journal Annals of Botany (2005) 96:625–638.
[5] Jackson, M.B., dan Colmer, T.D. “Response and adaptation by plants to flooding stress”. Ann. Bot. (2005) 96: 501-505.
[6] Jackson, M.B. “The impact of flooding stress on plants and crops”. School of Biological Sciences, University of Bristol, Bristol. (2003)pp. 1-15.
[7] Jackson, M.B., dan P.C. Ram. “Physiological and molecular basis susceptibility and tolerance of rice plants to comolete submergence”. Journal Annals of Botany (2003) 91:227-241.
[8] Hashiguchi, A., Ahsan, N., dan Komatsu, S. “Proteomics application of crops in the context of climatic changes”. Food Res. Int. (2010) 43: 1803-1813.
[9] Huang, S., Greenway, H., Colmer, T.D., dan Millar, A.H. “Protein synthesis by rice coleoptiles during prolonged anoxia: implications for glycolysis, growth and energy utilization”. Journal Annals of Botany (2005) 96:661-668.
[10]Ahmad, Parvaiz dan Salema Rasool, Emerging Technologies and Management of Crop Stress Tolerance. Sandiego: Elsevier 2014.
[11]Ahsan, N., Lee, D.G., Lee, S.H, Lee, K.W., Bahk, J.D., dan Lee, B.H. “A proteomic screen and identification of waterlogging-regulated proteins in tomato roots”. Journal Plant and Soil (2007) 295:37-51.
[12]Sach, M.M., Freeling, M., dan Okimoto, R. “The anaerobic proteins of maize”. Cell (1980) 20 : 761-767.
[13]Parent, C., Capelli, N., Berger, A., Crevecoeur, M., dan Dat, J.F. “An Overview of Plant Responses to Soil Waterlogging”. Journal of Plant Stress (2008) 2 : 20-27.
[14]Hurng, W.P., Huu, S.L., Cherng, K.L., dan Ching, H.K.. “Role of absisic acid, ethylene, and polyamines in flooding promoted senescence tobacco leaves”. Journal Plant Physiol (1993) 143: 102-105.
[15]Taiz, L dan E. Zeiger, Plant Physiology: Fifth Edition. Sunderland: Sinauer Associates, Inc. 2010.
[16]Hendriyani, I. K., dan N. Setiari. “Kandungan klorofil dan pertumbuhan kacang panjang (Vigna sinensis) pada tingkat penyediaan air yang berbeda”. Jurnal Sains & Mat, Vol. 17(3) (2009) 145-150.
[17]Kurniawan, Bayu Adi., Sisca, Fajriani., dan Ariffin. “Pengaruh jumlah pemberian air terhadap respon pertumbuhan dan hasil tanaman tembakau”.. Jurnal Produksi Tanaman (2014) 1 : 59-64.
[18]Laemmli, U.K. “Cleavage of structural protein during the assembly of the head of bacteriophage T4”. Nature (1970) 227: 680-685.
[19]Bollag, D.M., dan Edelstein. S.J, Protein Methods Department of Biochemistry. Switzerland: University of Geneva Press 1991.
[20] Durrani, Rabia., Abubakar, M., Arshed, M. Javed., Ullah, S.S.I., dan Ali, Qurban. “Biological characterization and protein profiles of two model bacteria by SDS-Page and FT-IR”. Journal of Agricultural and Biological Science Vol. 3 (5&6): (2008) 6-11.
[21]Stoychev, V., Lyudmila, S.S., Irina, V., Anelia, K.., Rosa, N., Urs, Feller., dan K. Demirevska. “Protein changes and proteolytic degradation in red and white clover plants subjected to waterlogging”. Acta Physiol Plant 2013.
[22]Komatsu, S., dan Hossain, Z. “Organ-spesific proteome analysis for identification of abiotic stress response mechanism in crop”. Frontiers in Plant Science Vol. 4 (2013).
[23]Meisrimler, C.N., Friedrich, B., dan Sabine, L. “Alterations in Soluble Class III Peroxidases of Maize Shoots by Flooding Stress”. Proteomes (2014) 2: 303-322.
[24]Lüthje, S., Möller, B., Perrineau, F.C., dan Wöltje, K.. “Plasma membrane electron pathways and oxidative stress”. Antioxid. Redox Signal (2013) 18: 2163–2183.
[25]Suzuki, N., Koussevitzky, S., Mittler, R., dan Miller, G. “ROS and redox signalling in the response of plants to abiotic stress”. Plant Cell Environ (2012) 35: 259–270.
[26]Chang, R., Jang, C. J., Branco-Price, C., Nghiem, P., dan Bailey-Serres, J.“Transient MPK6 activation in response to oxygen deprivation and reoxygenation is mediated by mitochondria and aids seedling survival in Arabidopsis”. Plant Mol. Biol (2012) 78: 109–122.

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