Antibacterial Activities Test of the Curcuminoid Compound in the Endophytic Bacteria of Curcuma zanthorrhiza Roxb.
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Nov 1, 2017
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Abstract
This study aims to determine the active compound curcuminoid contained in secondary metabolites 2 endophytic bacteria of temulawak (Curcuma zanthorrhiza Roxb.) rhizome BT2 and PD2 potentially as antibacterial compounds. TLC method used for separation of curcuminoid compounds followed by contact bioautography test to know the ability of compound activity which has been separated through TLC in inhibiting the growth of bacteria. The results of curcuminoid compound separation by TLC method are curcumin can be detected in BT2 and PD2 endophytic bacteria in the 4th spot with the same values of Rf 0.43. The desmetoxicurcumin fraction can be detected in BT2 and PD2 in the 3rd spot with the values of Rf 0.39 and 0.38 respectively. While bisdesmetoksikurkumin fraction can be detected in BT2 and PD2 in the 1st and 2nd spot with the value of Rf 0.25 and 0.32 respectively. Testing of contact bioautography on TLC plate containing separation of curcuminoid compounds produced by BT2 and PD2 doesn’t show any antibacterial activity.
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UTAMI, Ulfah; FUADATI, Choirul; PERDANA*, Terry Angria Putri.
Antibacterial Activities Test of the Curcuminoid Compound in the Endophytic Bacteria of Curcuma zanthorrhiza Roxb..
Proceedings of the International Conference on Green Technology, [S.l.], v. 8, n. 1, p. 300-305, nov. 2017.
ISSN 2580-7099.
Available at: <http://conferences.uin-malang.ac.id/index.php/ICGT/article/view/627>. Date accessed: 26 apr. 2024.
doi: https://doi.org/10.18860/icgt.v8i1.627.
Section
Biology
References
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[4] Hallman, J., Quadt-Hallman A., Mahaffee WF., Kloepper JW. 1997. Bacterial endophytes in agricultural crop. Can J Microbiol. 43(-):895-914
[5] Imawati, Rohana. 2015. Isolation and Identification of Endophytic Bacteria from Curcuma xanthorrhiza as a Producer of Antibacterial Compounds against Bacteria Pseudomonas aeruginosa and Staphylococcus epidermidis. Unpublished Thesis. Malang: UIN Malang.
[6] Inamandar, A.C and Pallit,A. 2003. The genus Malassezia ang human disease. Indian J.Dermatol Venereol Leprol
[7] Kusumaningtyas, E., Astuti E., and Darmono. 2008. Sensitivity method contact bioautography and agar overlay in determining compound antifungi. Indonesian Journal of Pharmaceutical Sciences. 6(2): 75-79
[8] Levy, S.B. 2002. Factors impacting on the problem of antibiotic resistence. J. of Antimicrobial Chemotheraphy. 49(-):25-30
[9] Listiandiani, Kirana. 2011. Identification of endophytic fungi ES1, ES2, ES3 and ES4 from Broussonetia papyrifera Vent. and antimicrobial activity test. Thesis. Depok: Department of Biology Faculty of Mathematics and Natural Sciences University of Indonesia.
[10] Lu, H. 1995. New bioactive metabolites produced by Colletrotricum sp. an endophytic fungus in Artemisia annua. Plant Sci. 151(-): 67-73
[11] Purnomowati, Sri. 2008. Benefits of Temulawak: A review of the literature of 1980 - 1997. http://www.indofarma.co.id/index.php?option=com_content&task=view&id=21&Itemid=125. (August 31, 2008)
[12] Rosana, Y., Sjahrurachman A., Setyaningsih ER. 1999. N. gonorrhoeae resistance study isolated from commercial sex workers in several places in Jakarta. J. Mikrobiol Indon. 4(-): 60-63
[13] Sastrohamidjojo, H. 2002. Chromatography Edisi II. Yogyakarta: Liberty.
[14] Sherma, J. and B. Fried. 2003. Handbook of Thin Layer Chromatography. New York: Marcel Dekker, Inc.
[15] Shibuya, H., Agusta A., Ohashi K., Maehara S., and Simanjuntak P. 2005. Biooxidation of (+)-catechin and (-)-epicatechin into 3,4-dihydroxy flavan derivatives by the endophytic fungus Diaporthe sp. isolated from a tea plant. Chem Pharm Bull. 53(7):866-867
[16] Siagian M.H. 2006. Temulawak as a plant Drugs and cultivation intensively. Bogor. Balitbang Botany, LIPI Research and Development Center.
[17] Stahl, E. 1985. Chromatographic and Microscopic Drug Analysis. Translated by: Kosasih Padmawinata and Iwang Soediro. Bandung: ITB
[18] Sudirman, L.I. 2005. Detection of Antimicrobial Compounds Isolated from Some Tropical Lentinus with Bioautographic Methods. Hayati. 12(2).
[19] Susanti, Ivy. 2008. NAD Provincial Government Launches International Year of Sanitation. Daily Alert; Waspada online 03 March 2008, accessed from http://www.waspada.co.id/. (March 3, 2008)
[20] Utami, U. 2011. Isolation, identification and antimicrobial activities selection of endophytic bacterial from mangrove Bruguiera gymnorrhiza. International Journal of Academic Research. 3(-):187-194
[21] Yulianty, R., Rante, H.,Alam,G. and tahir,A. 2011. Screening and analysis of TLC bioautography of antimicrobial compounds of several sponge extracts from the waters of Barrang Lompo Island, South Sulawesi. Traditional medicine magazine. 16(2):88-94.