POTENCY OF OIL SLUDGE INDIGENOUS BACTERIA FROM DUMAI-RIAU IN PRODUCING BIOSURFACTANT ON VARIATION OF SACCHARIDE SUBSTRATES

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Ni’matuzahroh Ni’matuzahroh Erta Tri Yuliawati Ditta Putri Kumalasari Nastiti Trikurniadewi Intan Ayu Pratiwi Salamun Salamun Fatimah Fatimah Sri Sumarsih Hanif Yuliani

Abstract

This study aims to detect the ability of four species of Dumai-Riau oil sludge indigenous bacteria to produce biosurfactants using 3 types of substrate (D-glucose, sucrose and molasses) and to determine the relationship between the five biosurfactant detection methods. The bacteria were grown on liquid mineral medium with addition of 2% saccharide substrates. The culture was incubated for 4 days at 300C. Production of biosurfactants by bacteria is detected in various ways by indirectly evaluating the presence of biosurfactant in the culture supernatant by measuring the surface tension (mN / m), emulsification (%), oil spreading, drop collapse and knowing the bacteria's ability to lysis blood agar media. The study used a completely randomized design with 4 treatments and 3 replications. The data were analyzed descriptively and statistically using Anova and continued with Duncan test and Pearson correlation test was performed to test the correlation between methods. The results showed the four bacteria gave different growth responses when grown on different substrates. The ability of bacteria to produce biosurfactants also varies depending on the type of substrates. The result of surface tension method has correlation with result of oil spreading method, blood agar test and emulsification activity.

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How to Cite
NI’MATUZAHROH, Ni’matuzahroh et al. POTENCY OF OIL SLUDGE INDIGENOUS BACTERIA FROM DUMAI-RIAU IN PRODUCING BIOSURFACTANT ON VARIATION OF SACCHARIDE SUBSTRATES. Proceedings of the International Conference on Green Technology, [S.l.], v. 8, n. 1, p. 339-346, nov. 2017. ISSN 2580-7099. Available at: <http://conferences.uin-malang.ac.id/index.php/ICGT/article/view/636>. Date accessed: 29 mar. 2024. doi: https://doi.org/10.18860/icgt.v8i1.636.
Section
Biology

References

Banat, I. M., Samarah, N., Murad, M., Horne, R., dan Banerjee, S., 1991, Biosurfactant Production and Use In Oil Tank Clean-Up, World Journal of Microbiology and Biotechnology 7(1): 80-88.

Batista, S. B., Mounteer, A. H., Amorim, F. R., dan Totola, M. R., 2006, Isolation and Characterization of Biosurfactant/Bioemulsifier-ProducingBacteria from Petroleum Contaminated Sites, Journal of Bioresource Technology 97(6): 868-875.

Desai, J. D. dan Banat, I. M., 1997, Microbial Production of Surfactants and Their Commercial Potential, Microbiology and Molecular Biology Reviews 61(1): 47-64.

Hogg, Stuart, 2005, Essential Microbiology, The University of Glamongan, UK.

Jain, D. K., Collins-Thomson, D. L., Lee, H., dan Trevors, J.T., 1991, A Drop-Collapsing Test for Screening Surfactant-Producing Microorganisms. Journal of Microbiological Methods 13(4): 271-279.

Karthik, L., Kumar, G., dan Rao, K.V.B., 2010, Comparison of Methods and Screening of Biosurfactant Producing Marine Actinobacteria

Katz, B. J., dan Dawson, W. C., 1997, A Structural and Tectonic Model of The Coastal Plain Block, Central Sumatra Basin, Indonesia, Proceedings of Indonesian Petroleum Association, 22th Annual Convention : 286-317p

Kurniasari, L., 2005, Pengolahan Awal Lumpur Minyak dengan Metode Volatilisasi. Momentum, 1(2).

Lin, S. C., 1996, Biosurfactants: Recent Advances. Journal of Chemical Technology and Biotechnology 66(2): 109-120.

Liu, W., Luo, Y.,Teng, Y., Li, Z., dan Ma, L. Q., 2010, Bioremediation of Oily Sludge-Contaminated Soil by Stimulating Indigenous Microbes, Enviromental geochemistry and health 32(1) :23-29

Morikawa, M., Hirata, Y., dan Imanaka, T., 2000, A Study on The Structure-Function Relationship of Lipopeptide Biosurfactants. Biochimicaet Biophysica Acta (BBA-Molecular and Cell Biology of Lipids 1488(3) : 211-218.

Mulligan, C. N., 2005, Environmental Applications for Biosurfactants, Environmental pollution 133(2) : 183-198.
Ni’matuzahroh, Alami, N.H., Khudlari, F., Fatimah, dan Tri, N., 2010, Studi Kinetika Produksi Biosurfaktan Bacillus subtilis 3KP pada Substrat Molase, Berkala Penelitian Hayati 16(33-38).

Ni’matuzahroh, Fatimah, Tini, S., Sri, S., 2013, “Biocleanoil” Produk Berbasis Mikroba untuk Pengolahan Sludge Industri Minyak, UPT Research Report Universitas Airlangga. Surabaya.

Ni’matuzahroh, Pratiwi, I.A., Irmayanti, I.S., Surtiningsih, T., Fatimah, Sumarsih, S., 2013, Combination of Acinetobacter sp. P2 (1) Biosurfactant and Crude Lipases on The Oil Sludge Solubility. Proceedings of The “4th International Conference & Workshop on Basic and Applied Sciences. lbnu Sina Institute for Fundamental Science Studies Universiti Teknologi Malaysia 81310 UTM Johor Bahru. 172-177.671-674.

Ni’matuzahroh, Puspitasari, A. O., Pratiwi, I.A., Fatimah, Sumarsih, S., Surtiningsih, T., Salamun., 2015. Oil Removal from Petroleum Sludge using Bacterial Culture with Molasses Substrate at TemperatureVariation. Proceedings 5th International Conference and Workshop on Basic and Applied Sciences (ICOWOBAS 2015) AIP Conf. Proc. 1718, 030002-1–030002-8; doi: 10.1063/1.4943312 © 2016 AIP Publishing LLC 978-0-7354-1364-1

Ni’matuzahroh, Trikurniadewi, N., Pramadita A. R. A. , Pratiwi I. A.Salamun, Fatimah, Sumarsih, S. 2017. Biodegradation of Naphthalene and Phenanthrene by Bacillus subtilis 3KP. Proceedings 3rd International Biology Conference (IBOC) and 10 th KOREA-ASEAN Biomass Symposium: Biodiversity and biotechnology for human walfare. AIP Conf. Proc.1854.020026. AIP Publishing ISBN : 978-0-7354-1528-7

Pruthi, V. dan Cameotra, S. S., 1997, Rapid Identification of Biosurfactant-Producing Bacterial Strains Using a Cell Surface Hydrophobicity Technique, Biotechnology Techniques, 11(9):

Shah, N., Nikam, R., Gaikwad, S., Sapre, V., dan Kaur, J, 2016, Biosurfactant: Types, Detection Methods, Importance and Applications. Indian J Microbial Res 2016 3(1):5-10.

Singh, V., 2012, Biosurfactant - Isolation, Production, Purification and Significance, Int J Sci Res Pub, 2(7):1-4.

Suastuti, N. G. A. M. A., 1998, Pemanfaatan Hasil Samping Industri Pertanian (Molase dan Limbah Cair Tahu) sebagai Sumber Karbon dan Nitogen untuk Produksi Biosurfaktan oleh Bacillus sp. Galur Komersial dan Lokal, Institut Pertanian Bogor.

Suryatmana, P. E. Kardena, E. Ratnaningsih, dan Wisjnuprapto, 2006, Karakteristik Biosurfaktan dari Azotobacter chroococcum. Jurnal Microbiologi Indonesia. 2(1): 30-34.

Walter, V., Syldatk, C. dan Hausmann, R., 2010, Screening Concepts for The Isolation of Biosurfactant Producing Microorganisms, Biosurfactants (pp. 1-13). Springer New York.

Youssef, N. H., Duncan, K. E., Nagle, D. P., Savage, K. N., Knapp, R. M., dan McInerney, M. J., 2004, Comparison of Methods to Detect Biosurfactant Production by Diverse Microorganisms, Journal of Microbiological Methods 56(3): 339-347.

Zahroh, Fatimatuz, 2011, Pengaruh konsentrasi Gula Cair dan Waktu Inkubasi Terhadap Produksi Biosurfaktan Bacillus subtilis 3KP, Skripsi, Universitas Airlangga, Surabaya.

Zaragoza, A., Fransisco J.A., Maria J.E., Jose A.T., Ana M., Angeles M. dan Antonio O., 2010, Hemolytic Activity of a Bacterial Trehalose Lipid Biosurfactant Produced by Rhodococcus sp.: Evidence for a Colloid-Osmotic Mechanism, Langmuir 26(11): 8567-8572.