The Effect of Schleichera oleosa L. on HbA1c Levels in Alloxan-Induced Rats


Diabetes mellitus is a chronic disease that occurs due to the failure of the pancreas to produce sufficient insulin, or the body cannot use the insulin it makes effectively. The HbA1c (glycated hemoglobin) levels show an alteration in blood glucose levels in people with diabetes. As a traditional medicine, the kesambi plant (Schleichera oleosa L.) can improve diabetic people's health due to its secondary compounds, such as alkaloids, flavonoids, tannins, steroids, and phenolic compounds, besides several empirically proven as antiulcer, anticancer, and antimicrobial effects. The study aimed to determine the effect of the ethanol extract of Kesambi leaves on HbA1c levels in alloxan-induced rats. This research used a posttest-only design method, using 18 rats divided into six groups of mice and induced by alloxan 150 mg/kg in intraperitoneal with the division of groups: group I without treatment; group II alloxan + Na CMC; group III alloxan + glibenclamide; groups IV, V, and VI, given alloxan + kesambi leaf extract 200, 400, and 600 mg/kg BW, respectively. All treatments were given orally for 23 days. HbA1c values were measured on day 24 using the Afinion HbA1c. The data obtained were analyzed using the method of comparing the measurement results of each group against the control group. The results showed that the 200, 400, and 600 mg extracts affected HbA1c levels with successive values of 5.47%, 5.37%, and 5.47% compared to the control group, namely 5.10%. This study concluded that giving kesambi leaf ethanol extract can affect HbA1c levels.

Author Biographies

Akbar Awaluddin, Sekolah Tinggi Ilmu Farmasi Makassar

Department of Pharmacology and Clinical Pharmacy, Sekolah Tinggi Ilmu Farmasi Makassar, Jalan Perintis Kemerdekaan Km.13,7 Daya, Makassar, 90242, Indonesia; +6285345588048

Andi Zulkifli AS, Universitas Megarezky

Diploma IV Medical Laboratory Technology, Universitas Megarezky, Jalan Antang Raya, Antang, Makassar, 90234, Indonesia; +6285397119122

Asril Burhan, Sekolah Tinggi Ilmu Farmasi Makassar

Department of Biological Pharmacy, Sekolah Tinggi Ilmu Farmasi Makassar, Jalan Perintis Kemerdekaan Km.13,7 Daya, Makassar, 90242, Indonesia; +628116188155

Nurhikma Awaluddin, Universitas Megarezky

Department of Pharmacology and Clinical Pharmacy, Universitas Megarezky, Jalan Antang Raya, Antang, Makassar, 90234, Indonesia; +6282122291122

Hamka Amir, Sekolah Tinggi Ilmu Kesehatan Tanawali

Department of Nursing, Sekolah Tinggi Ilmu Kesehatan Tanawali, Jl. Diponegoro, Bajeng, Takalar, 92210, Indonesia; +6281286416311


Abubakar, A. R. & Haque, M. (2020). Preparation of Medicinal Plants: Basic Extraction and Fractionation Procedures for Experimental Purposes. Journal of Pharmacy and Bioallied Sciences, 12(1), 1-10.
American Diabetes Association. (2010). Diagnosis and Classification of Diabetes Mellitus. Diabetes Care, 33(Supplement_1), S62-S69.
Borges, A., José, H., Homem, V., & Simões, M. (2020). Comparison of Techniques and Solvents on the Antimicrobial and Antioxidant Potential of Extracts from Acacia dealbata and Olea Europaea. Antibiotics, 9(2), 48.
Dharmojono. (2010). Penyakit - Penyakit Pada Anjing dan Kucing dan Permasalahannya. Edisi Kedua. Jakarta: Dharma Scientifiec Books.
Dias, M. C., Pinto, D. C. G. A., & Silva, A. M. S. (2021). Plant Flavonoids: Chemical Characteristics and Biological Activity. Molecules, 26(17), 5377.
Eddouks, M., Chattopadhyay, D., & Zeggwagh, N. A. (2012). Animal models as tools to investigate antidiabetic and anti-inflammatory plants. Evidence-Based Complementary and Alternative Medicine, 2012, 142087.
Finkel, R., Harvey, R. A., Champe, P. C., Ramadhani, D., Tjahyanto, A., Salim, C., et al. (2013). Farmakologi Ulasan Bergambar Edisi 4. Jakarta: Buku Kedokteran EGC.
Goldstein, D., Wiedmeyer, H. M., England, J. D., Little, R. R., & Parker, K. M. (1984). Recent advances in glycosylated hemoglobin measurements. Critical Reviews in Clinical Laboratory Sciences, 21(3), 187-228.
Goswami, S. & Singh, R. P. (2019). Antidiabetic Potential and HPTLC. Fingerprinting of Schleichera oleosa (Lour) Oken, Pharmacognosy Journal, 11(3), 469-474.
Kumar, A. Y., Nandakumar, K., Handral, M., Talwar, S., & Dhayabaran, D. (2011). Hypoglycaemic and anti-diabetic activity of stem bark extracts Erythrina indica in normal and alloxan-induced diabetic rats. Saudi Pharmaceutical Journal, 19(1), 35-42.
Muthukrishnan, S. & Sivakkumar, T. (2017). In Vitro Studies to Assess the Antidiabetic Potential of Schleichera oleosa (Lour) Oken Leaves. Asian Journal of Pharmaceutical and Clinical Research, 10(3), 280-283.
PERKENI. (2015). Konsensus pengelolaan dan pencegahan diabetes melitus tipe 2 di indonesia 2011. Jakarta: PB PERKENI.
Shambaditya, G., Pal, S. R., & Ritu, G. M. (2019). Comparative effect of chloroform extracts of Schleichera oleosa and Tagetes erecta on Ascardia galli. In 7th INDO US Conference 2019. Swami Keshvanand College of Pharmacy, Jaipur, India, 37.
Sherwani, S. I., Khan, H. A., Ekhzaimy, A., Masood, A., & Sakharkar, M. K. (2016). Significance of HbA1c Test in Diagnosis and Prognosis of Diabetic Patients. Biomarker Insights, 11, 95-104.
Situmeang, B., Nuraeni, W., Ibrahim, A. M., & Silaban, S. (2016). Analysis of secondary metabolite compounds from leaves extract kesambi (Schleichera oleosa) and antioxidant activity test. Jurnal Pendidikan Kimia (JPKIM), 8(3), 164-168.
Subronto. (2006). Penyakit Infeksi Parasit Mikroba & Metabolisme pada Anjing & Kucing. Yogyakarta: Gadjah Mada University Press.
Wang, T., Li, X., Zhou, B., Li, H., Zeng, J., & Gao, W. (2015). Anti-diabetic activity in Type 2 diabetic mice and α-glucosidase inhibitory, antioxidant and anti-inflammatory potential of chemically profiled pear peel and pulp extracts (Pyrus Spp.). Journal of Functional Foods, 13, 276-288.
Zhang, Q. W., Lin, L. G., & Ye, W. C. (2018). Techniques for extraction and isolation of natural products: a comprehensive review. Chinese Medicine, 13, 20.
How to Cite
AWALUDDIN, Akbar et al. The Effect of Schleichera oleosa L. on HbA1c Levels in Alloxan-Induced Rats. Proceedings of International Pharmacy Ulul Albab Conference and Seminar (PLANAR), [S.l.], v. 2, p. 1-7, dec. 2022. ISSN 2827-7848. Available at: <>. Date accessed: 30 sep. 2023. doi: