Determination of Quercetin in Extracts and Herbal Products of Phyllanthus niruri by TLC Densitometry Method
Abstract
Phyllanthus niruri has many pharmacological activities such as antioxidant, anti-inflammatory and anticancer. Phyllanthus niruri has been widely developed from extracts into herbal products. The flavonoid quercetin became an analytical marker compound to ensure the quality of P. niruri extracts in the Indonesian herbal pharmacopeia II. This study aimed to determine the levels of quercetin in extract and herbal products using the TLC Densitometry method. TLC Densitometry method using chloroform: ethyl acetate: formic acid (5:4:1) as the mobile phase and silica GF254 as the stationary phase. The Rf value of the quercetin compound obtained is 0.50. The Rs quercetin is 1.56 in the extract and 2.55 in the product. The maximum wavelength for quercetin is 366 nm. The results of quercetin levels were 3.579% in the extract and 0.0412% in herbal products. The TLC densitometry method can analyze quercetin in extracts and herbal products.
References
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Shanbhag, T., Amuthan, A., Shenoy, S., & Sudhakar. (2010). Effect of Phyllanthus niruri. Linn on burn wound in rats. Asian Pacific Journal of Tropical Medicine, 3(2), 105–108. https://doi.org/10.1016/S1995-7645(10)60045-4
Soylak, M., Ozdemir, B., & Yilmaz, E. (2020). An environmentally friendly and novel amine-based liquid phase microextraction of quercetin in food samples prior to its determination by UV–vis spectrophotometry. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 243, 118806. https://doi.org/10.1016/j.saa.2020.118806
Zhang, Q. W., Lin, L. G., & Ye, W. C. (2018). Techniques for extraction and isolation of natural products: a comprehensive review. Chinese Medicine, 13, 20. https://doi.org/10.1186/s13020-018-0177-x
Amir, M. Mujeeb, M., Ahmad, S., Akhtar, M., & Ashraf, K. (2013). Design expert-supported development and validation of HPTLC method: An application in simultaneous estimation of quercetin and rutin in Punica granatum, Tamarindus indica and Prunus domestica. Pharmaceutical Methods, 4(2), 62–67. https://doi.org/10.1016/j.phme.2013.12.004
Bagalkotkar, G., Sagineedu, S. R., Saad, M. S., & Stanslas, J. (2010). Phytochemicals from Phyllanthus niruri Linn. and their pharmacological properties: a review. Journal of Pharmacy and Pharmacology, 58(12), 1559–1570. https://doi.org/10.1211/jpp.58.12.0001
Colpo, E., Vilanova, C. D. D. A., Pereira, R. P., Reetz, L. G. B., Oliveira, L., Farias, I. L. G., et al. (2014). Antioxidant effects of Phyllanthus niruri tea on healthy subjects. Asian Pacific Journal of Tropical Medicine, 7(2), 113–118. https://doi.org/10.1016/S1995-7645(14)60005-5
Dinakaran, S. K., Sujiya, B., & Avasarala, H. (2018). Profiling and determination of phenolic compounds in Indian marketed hepatoprotective polyherbal formulations and their comparative evaluation. Journal of Ayurveda and Integrative Medicine, 9(1), 3–12. https://doi.org/10.1016/j.jaim.2016.12.006
Hafid, A. F., Rifai, B., Tumewu, L., Widiastuti, E., Dachliyati, L., Primaharinastiti, R., et al. (2015). Andrographolide determination of Andrographis paniculata extracts, ethyl acetate fractions and tablets by thin layer chromatography. Journal of Chemical and Pharmaceutical Research, 7(12), 557-561.
Ifeoma, O., Samuel, O., Itohan, A. M., & Adeola, S. O. (2013). Isolation, fractionation and evaluation of the antiplasmodial properties of Phyllanthus niruri resident in its chloroform fraction. Asian Pacific Journal of Tropical Medicine, 6(3), 169–175. https://doi.org/10.1016/S1995-7645(13)60018-8
Ihsan, B. R. P., Hafid, A. F., Primaharinastiti, R., Widyawaruyanti, A., & Yuwono, M. (2020). Development and validation of hplc method for determination of andrographolide in raw material and tablet of ethyl acetate fractions of Andrographis paniculata. Research Journal of Pharmacy and Technology, 13(9), 4291. https://doi.org/10.5958/0974-360X.2020.00758.1
Ihsan, B. R. P., Maysaroh, I., & Nurhayati, I. P. (2018). Validasi Metode Ultra High Performance Chromatography Double Mass Spectrometry (UHPLC-MS/MS) untuk Analisis Kurkumin pada Ekstrak Etanol Kunyit (Curcuma longa) dengan Berbagai Perbandingan. Pharmaceutical Journal of Indonesia, 4(1), 29-34.
Ihsan, B. R. P., Rahmani, P. A., & Shalas, A. F. (2019). Validasi Metode KLT-Densitometri untuk Analisis Kuersetin dalam Ekstrak dan Produk Jamu yang Mengandung Daun Jambu Biji (Psidium guajava L.). Pharmaceutical Journal of Indonesia, 5(1), 45-51.
Kumar, S., Chandra, P., Bajpai, V., Singh, A., Srivastava, M., Mishra, D. K., et al. (2015). Rapid qualitative and quantitative analysis of bioactive compounds from Phyllanthus amarus using LC/MS/MS techniques. Industrial Crops and Products, 69, 143–152. https://doi.org/10.1016/j.indcrop.2015.02.012
Nutmakul, T. (2022). A review on benefits of quercetin in hyperuricemia and gouty arthritis. Saudi Pharmaceutical Journal, 30(7), 918–926. https://doi.org/10.1016/j.jsps.2022.04.013
Pathania, R., Najda, A., Chawla, P., Kaushik, R., & Khan, M. A. (2022). Low-energy assisted sodium alginate stabilized Phyllanthus niruri extract nanoemulsion: Characterization, in vitro antioxidant and antimicrobial application. Biotechnology Reports, 33, e00711. https://doi.org/10.1016/j.btre.2022.e00711
Porto, C. R. C., Soares, L. A. L., Souza, T. P., Petrovick, P. R., Lyra, I. L., Junior, R. F. A., et al. (2013). Anti-inflammatory and antinociceptive activities of Phyllanthus niruri spray-dried standardized extract. Revista Brasileira de Farmacognosia, 23(1), 138–144. Available at: https://doi.org/10.1590/S0102-695X2013005000004
Seyed, M. A. (2019). A comprehensive review on Phyllanthus derived natural products as potential chemotherapeutic and immunomodulators for a wide range of human diseases. Biocatalysis and Agricultural Biotechnology, 17, 529–537. https://doi.org/10.1016/j.bcab.2019.01.008
Shanbhag, T., Amuthan, A., Shenoy, S., & Sudhakar. (2010). Effect of Phyllanthus niruri. Linn on burn wound in rats. Asian Pacific Journal of Tropical Medicine, 3(2), 105–108. https://doi.org/10.1016/S1995-7645(10)60045-4
Soylak, M., Ozdemir, B., & Yilmaz, E. (2020). An environmentally friendly and novel amine-based liquid phase microextraction of quercetin in food samples prior to its determination by UV–vis spectrophotometry. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 243, 118806. https://doi.org/10.1016/j.saa.2020.118806
Zhang, Q. W., Lin, L. G., & Ye, W. C. (2018). Techniques for extraction and isolation of natural products: a comprehensive review. Chinese Medicine, 13, 20. https://doi.org/10.1186/s13020-018-0177-x
Published
2022-12-05
How to Cite
IHSAN, Bachtiar Rifai Pratita; DELINA, Ade Putri; SHALAS, Alvan Febrian.
Determination of Quercetin in Extracts and Herbal Products of Phyllanthus niruri by TLC Densitometry Method.
Proceedings of International Pharmacy Ulul Albab Conference and Seminar (PLANAR), [S.l.], v. 2, p. 31-37, dec. 2022.
ISSN 2827-7848.
Available at: <http://conferences.uin-malang.ac.id/index.php/planar/article/view/1855>. Date accessed: 09 may 2024.
doi: https://doi.org/10.18860/planar.v2i0.1855.
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