Genetic Variation of Porang (Amorphophallus muelleri Blume) After Mutation Induction Using Colchicine
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Published
Dec 31, 2024
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Abstract
Porang (Amorphophallus muelleri Blume) is a tuberous plant with the highest glucomannan among Amorphophallus genera. However, the low genetic diversity of porang today can affect porang cultivation in the future. Therefore, increasing the genetic diversity of porang by polyploidy induction using colchicine is needed. 30 days after planting porang shoot explants were used for polyploidy induction. After polyploidy induction using colchicine, DNA was extracted using Geneid Genomic DNA Mini Kit. Genetic diversity and clustering analysis was performed to assess the genetic diversity of porang after mutation induction. Mutation induction using colchicine successfully increased the genetic diversity of porang. Polyploidy induction using 0.05% colchicine is recommended to increase genetic variation of porang.
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How to Cite
WAHYUDI, Didik et al.
Genetic Variation of Porang (Amorphophallus muelleri Blume) After Mutation Induction Using Colchicine.
Proceedings of the International Conference on Green Technology, [S.l.], v. 14, n. 1, dec. 2024.
ISSN 2580-7099.
Available at: <https://conferences.uin-malang.ac.id/index.php/ICGT/article/view/3224>. Date accessed: 07 feb. 2026.
Section
Biology
References
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[37] Yulianti F., C Martasari, Karsinah, and Tangguh Hartanto. 2010. Variasi Genetik Jeruk Keprok SoE (Citrus reticulate Blanco) Hasil Radiasi Sinar Gamma Menggunakan Penanda ISSR. Bul. Plasma Nutfah. 16(2).
[2] Widarta IWR., Ambar R, Umar S, Supriyadi, and Sri R. 2022. Optimization of Oil-In-Water Emulsion Capacity and Stability of Octenyl Succinic Anhydride-Modified Porang Glucomannan (Amorphophallus muelleri Blume). Research Article Heliyon.
[3] Poerba YS and Diyah M. 2008. Keragaman Genetik Berdasarkan Marka Random Amplifies Polymorphic DNA pada Amorphophallus muelleri Blume di Jawa. Jurnal Biodiversitas. 9(4):245-249.
[4] Setiawati E., Bahri S, and Razak AR. 2017. Ekstraksi Glukomanan dari Umbi Porang (Amorphophallus paeniifolius (Dennst.) Nicolson). J. Kovalen. 3(3):234-241.
[5] Sulistyo RH., Lita S, and Damanhuri. 2015. Eksplorasi dan Identifikasi Karakter Morfologi Porang (Amorphophallus muelleri Blume) di Jawa Timur. J. Produksi Tanaman. 3(5):353-361.
[6] Nugrahaeni N., Ratri TH, TRustinah, Febria CI, Sutrisno, Amri A, Eriyanto Y, Siti M, Yuliantoro B, and Joko SU. 2021. Morphological Characteristics of Madiun 1, the First Porang (Amorphophallus muelleri Blume) Released Cultivar in Indonesia. Int. Conf. Earth and Enviromental Sci..
[7] Sharma S and Neeraj W. 2022. Application of Glucomannan. Rev. Artic. J. of Pharmaceutical Res. 21:1-5.
[8] Kamsiati ES., Widowati, and H Herawati. 2022. Utilization of Porang Flour Producing Tapioca Based Gluten-Free Noodles and Characteristics of the Product. Int. Conf. on Agric. Postharvest Hand. and Process.
[9] Setyadi ERF., Amani SH, and Setiyo G. 2022. Pra Desain Pabrik Konnyaku dari Umbi Porang (Amorphophallus oncophyllus) dengan Metode Kombinasi Hidrolisa Enzim α-Amilase dan Ekstraksi Etanol. J. Teknik ITS. 11(2).
[10] Sarifudin A., Lia R, Novita I, Riyanti E, Enny S, Nok A, Dewi D, Pramono N, and Annisa DY. 2022. Evaluation of Some Analytical Methods for Determination of Calcium Oxalate in Amorphophallus muelleri Flour. Original Article Food Sci. and Technol..
[11] Trianto S., Tri NP, and Fadilah. 2018. Studi Pembuatan Sposs Dari Tepung Porang Kuning (Amorphophallus muelleri Blume). Semin. Nas. Teknik Kimia Ecosmart.
[12] Kurniawati YI., Bagus PYK, Muksin, and Andarula G. 2022. Strategi Pengembangan Bisnis Bibit Porang Jember. J. Ilm. Inov. 22(2).
[13] Padusung F., Mahrup, Kusnarta, and Soemeinaboedhy. 2020. Meningkatkan Kesejahteraan Petani Hutan Melalui Integrasi Tanaman Porang (Amorphophallus onchophyllus) dengan Vegetasi Tegakan di Kawasan Rinjani Lombok. Pros. Semin. Nas. Pertan.
[14] Supriati Y.2016. Keanekaragaman Iles-Iles (Amorphophallus spp.) dan Potensinya Untuk Industri Pangan Fungsional, Kosmetik, dan Bioetanol. J. Litbang Pertan. 35(2):69-80.
[15] Lestari, Endang Gati. 2021. Aplikasi Induksi Mutasi Untuk Tanaman Hias. J. Ilmu-Ilmu Hayati.
[16] Gultom, Tumiur. 2016. Pengaruh Pemberian Kolkisin Terhadap Jumlah Kromosom Bawang Putih (Allium sativum) Lokal Kultivar Doulu. J. Biosains. 2(3).
[17] Firmansyah BF., Saptadi D, and Ardiarini NR. 2019. Evaluasi Keragaman (Vigna Subterranean L.) Verdcort) Hasil Induksi Mutasi Kolkisin. J. Produksi Tanaman. 7(1):53-59.
[18] Wiendra NMS., Made P, and Ni Putu AA. 2011. Pemberian Kolkisin dengan Lama Perendaman Berbeda pada Induksi Poliploidi Tanaman Pacar Air (Impatiens balsamina). Jurnal Biologi. 15(1):9-14.
[19] Sirojuddin TR and Saimul L. 2017. Pengaruh Pemberian Berbagai Konsentrasi Kolkisin dan Lama Perendaman Terhadap Respon Fenotipik Zaitun (Olea europaea). J. Ilm. Biosaintropis. 2(2):36-41.
[20] Dewi IARP and Pharmawati M. 2018. Penggandaan Kromosom Marigold (Tagetes erecta L.) dengan Perlakuan Kolkisin. Majalah Ilmiah Biosfera : A Scientific J. 35(3):152-157.
[21] Ainurrohman C and Isnawati. 2020. Perbandingan Efektivitas Ekstrak Etanolik Umbi Kembang Sungsang (Gloriosa superba L.) dan Daun Tapak Dara (Catharanthus roseus) Sebagai Subtitusi Kolkisin. J. LenteraBio. 9(2):158-167.
[22] Djuita NR., Alex H, Tatik C, and Dorly. 2020. Keanekaragaman Genetik Kapulasan [Nephelium Ramboutan-Ake (Labill.) Leenh.] Di Jawa Berdasarkan Marka SSR dan ISSR. J. Sistematika Tumbuhan Floribunda. 6(4).
[23] Aprilianingsish R., Baiq FW, and Muhammad RH. 2021. Optimasi Suhu Annealing Marka ISSR Sebagai Langkah Awal Pengejawantahan Keragaman Genetik Ficus fistulosa dan Ficus variegate. Gunung Djati Conference Series.
[24] Buhroo ZI., MA Bhat, NA Ganai, AS Kamili, GK Bali, and A Aziz. 2018. An Efficient Protocol for The Inter-Simple Sequence Repeat (ISSR) Marker Approach in Population Genetic Studies. J. of Entomology and Zoology Stud. 6(4):597-600
[25] Solikin A., Retnoningsih A, and Rahayu ES. 2017. Karakterisasi Aksesi Durian Lokal Koleksi Hortimart Agro Centre Jawa Tengah Menggunakan Penanda Molekuler Inter Simple Sequence Repeats (ISSR). J. Floribunda. 5(7).
[26] Zvygina NS., Olga VD, and Alexander AK. 2015. Genetic Differentiation and Karyotype Variation in Hedysarm chaiyrakanicum, an Endemic Species of Tuva Republic, Russia. Indian J. of Exp. Biol. 54: 338-334.
[27] Sica M., Graziella G, Stefania M, Luciano G, and Serena A. 2005. ISSR Markers Shoow Differentiation Among Italian Population of Asparagus acutifolius L. Res. Artic. BMC Genetics.
[28] Hu J., Xiaoxi G, Jun L, Conghua X, and Jianwu L. 2008. Plant Regeneration From Petiole Callus of Amorphophallus albus and Analysis of Somaclonal Variation of Regenerated Plants by RAPD and ISSR Markers. Botanical Stud. 49: 189-197.
[29] Madani H, Escrich A, Hosseini B, Sanchez-Muñoz R, Khojasteh A, Palazon J. Effect of Polyploidy Induction on Natural Metabolite Production in Medicinal Plants. Biomolecules. 2021 Jun 17;11(6):899. doi: 10.3390/biom11060899. PMID: 34204200; PMCID: PMC8234191.
[30] Madlung, A. Polyploidy and its effect on evolutionary success: old questions revisited with new tools. Heredity 110, 99–104 (2013). https://doi.org/10.1038/hdy.2012.79
[31] Govindaraj M, Vetriventhan M, Srinivasan M. Importance of genetic diversity assessment in crop plants and its recent advances: an overview of its analytical perspectives. Genet Res Int. 2015;2015:431487. doi: 10.1155/2015/431487. Epub 2015 Mar 19. PMID: 25874132; PMCID: PMC4383386.
[32] Trimanto. 2012. Karakterisasi dan Jarak Kemiripan Uwi (Dioscorea alata L) Berdasarkan Penanda Morfologi Umbi. Buletin Kebun Raya. 15(1): 46-55.
[33] Archetti, Marco. 2022. Polyploidy as an Adaptation Againts Loss of Heterozygosity in Cancer. International J. of Mol. Sci.
[34] Kafer, Etta. 1986. Test Which DDistinguish Induced Crossing-Over and Aneuploidy from Secondary Segregation in Aspergillus Treated with Hydrate or γ-Rays. Mutation Research.
[35] Gao, C., Guilong Z, Chaozhi M, Wen Z, Tong Z, Zhiquan L, Yong Y, Ming W, Yao Y, Zhiqiang D, Yaya L, Bing L, Jinxiong S, Jinxing T, and Tingdong F. 2016. Helitron- like Transposons Contributed to The Mating System Transition From Out-Crossing to Self-Fertilizing in Polyploid Brassica napus L. Scientific Reports.
[36] Senchina DS., Ines A, Richard CC, Bao L, Junkang R, Richard DN, Andrew HP, Rod AW, Thea AW, and Jonathan F. 2003. Wendel. Rate Variation Among Nuclear Genes and The Ages of Polyploidy in Gossypium. Mol. Bio. 20(4): 633-643.
[37] Yulianti F., C Martasari, Karsinah, and Tangguh Hartanto. 2010. Variasi Genetik Jeruk Keprok SoE (Citrus reticulate Blanco) Hasil Radiasi Sinar Gamma Menggunakan Penanda ISSR. Bul. Plasma Nutfah. 16(2).