OPTIMIZATION OF CELLULASE PRODUCTION BY CANDIDA G3.2 FROM THE RHIZOSPHERE OF GUNUNG ANYAR MANGROVE SURABAYA
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Published
Nov 1, 2017
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Abstract
Microbial cellulases are the enzymes widely used in many biochemical industries. Yeast has potential to degrade cellulose because of cellulase activity. Yeast from the genus of Candida G3.2 has the ability to hydrolaze cellulose which tested by cellulolytic index. The purpose of this study is to determine the incubation time and the combination of physicochemical factors (pH and temperature) optimal for the production of cellulase enzymes by yeasts.The study design by factorial which analyzed by descriptive quantitative. This study begins with the optimization of cellulase production, starting with the optimization of the incubation period and followed by a combination of temperature optimization (room, 45°C, 55°C) and pH (4.5, 6, 8) then measure the cellulase activity. The results showed that the best incubation period for cellulase production is 72 hours (267 U/ml) with a combination of temperature 55°C and pH 6 (369.8 U/ml).
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How to Cite
ALAMI, N. H. et al.
OPTIMIZATION OF CELLULASE PRODUCTION BY CANDIDA G3.2 FROM THE RHIZOSPHERE OF GUNUNG ANYAR MANGROVE SURABAYA.
Proceedings of the International Conference on Green Technology, [S.l.], v. 8, n. 1, p. 399-406, nov. 2017.
ISSN 2580-7099.
Available at: <http://conferences.uin-malang.ac.id/index.php/ICGT/article/view/655>. Date accessed: 26 apr. 2024.
doi: https://doi.org/10.18860/icgt.v8i1.655.
Section
Biology
References
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Agbogbo, F.K., Hagensen, F.D., Milam, D., dan Wenger, K. S. 2008. Fermentation Of Acid-Pretreated Corn Stover To Ethanol Without Detoxification Using Pichia stipitis. Journal of Application Biochemical Biotechnology 145:53–58
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Asaduzzaman. 2007. Standardization of Yeast Groth Curve from Several Curves with Different Initial Sizes. Tesis. Gȍteborg. Departement of Mathematical Statistics, Chalmers University of Technology and Gȍteborg University
Ates, S., Elibol, M., Mavituna, F. 1997. Production of Actinorhodin by Streptomyces coelicolor in Batch and Fed Batch Cultures. Journal of Process Biochemistry 32:273-278
Ballesteros, M., Olivia, J.M., Negro, M.J., Manzanares, P., Ballesteros, I. 2004. Ethanol from Lignocellulosic Materials by a Simultaneous Saccharification and Fermentation Process (SFS) with Kluyveromyces marxianus CECT 10875. Journal of Process Biochemichal 39:1843-1848
Chasanah, E., Dini, I.R., Mubarik, N.R. 2013. Karakterisasi Enzim Selulase PMP 1026Y dari Limbah Pengolahan Agar. Jurnal JPB Perikanan 8:103-114
Chi, Z., Zhang, T., Liu, G., Li, J., Wang, X. 2009. Production, Characterization And Gene Cloning Of The Extracellular Enzymes From The Marine-Derived Yeasts And Their Potential A:lications. Journal of Biotechnology Adv 27:236–255
Choder M. A. 1991. General Topoisomerase I-Dependent Transcriptional Repression In The Stationary Phase In Yeast. Genes Dev. 5:2315–2326.
Das, A., Paul, T., Halder, S.K., Maity, C., Mohapatra. P. K. D., Pati, B. R., Mondal, K.C. 2013. Study on Regulation of Growth and Biosynthesis of Cellulolytic Enzymes from Newly Isolated Aspergillus fumigatus ABK9. Journal of Microbiology 62:31-43
Englard, S., dan Sifters, S. 1990. Precipitation Techniques. Journal of Methods Enzymology 182:285-300
Fardiaz, S. 1992. Mikrobiologi Pangan I. Jakarta:Gramedia Pustaka Utama.
Ferreira, S.M.P., Duarte, A.P., Queiroz, J.A., dan Domingues, F.C. 2009. Influence of Buffer Systems on Trichoderma reesei Rut C-30 Morphology and Cellulase Production. Electronic Journal of Biotechnology 12:3
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Harshvardhan, K.., Mishr, A., Jha, B. 2013. Purification and Characterization of Cellulase from A Marine Bacillus sp. H1666: A Potential Agent For Single Step Saccharification Of Seaweed Biomass. Journal of Molecular Catalysis B: Enzymatic 93:51-56
Ijaz, A., Anwar, Z., Zafar, Y., Hussain, I., Muhammad, A., Irshad, M., Mehmood, S. 2011. Optimization of Cellulase Enzyme Production from Corn Cobs Using Alternia alternata by Solid State Fermentation. Journal of Cell and Molecular Biology 9 : 51-56
Ire, F. S., Okolo, B. N., Moneke, A. N., Odibo, F. J. C. 2011. Influence of Cultivation Conditions on The Production of A Protease From Aspergillus carbonarius Using Submerged Fermentation. African Journal of Food Science 5:353-365
Johri, B.N., Alluralde, J.D., Klein, J. 1990. Lipase Production by Free and Immobilized Protoplasts Sporotrichum thermophile. Journal of Applied Microbiology and Biotechnology 33:367-371
Kadam, K.L., Schmidt, S.L. 1997. Evaluation of Candida acidothermophilus in Ethanol Production from Lignocellulosic Biomass. Journal of A.l.Microbiol. Biotechnol 48 : 709-713.
Kathiresan, K., Saravanakumar, K., Senthilraja, P. 2011. Bioethanol Production by Marine Yeast Isolated from Coastal Mangrove Sediment. Journal of Int. Multidiscip Res 1 : 19-24
Kirk, O., Borchert, T. V., and Fuglsang, C. C. 2002. Industrial enzyme a:lications. Journal of Curr. Opin. Biotechnol 13 : 345-351
Kurtzman, C.P. dan Fell, J.W. 1998. The Yeasts a Taxonomic Study, 4th edn. Amsterdam:Elsevier
Lehninger, A.L. 2000. Dasar-Dasar Biokimia. Jakarta: Erlangga
Lu, W., Li, D., Wu, Y. 2003, Influence of Water Activity and Temperature on Xylanase Biosynthesis in Pilotscale Solidstate Fermentation by Aspergillus sulphurous. Journal of Enzyme Microbiol. Technology 32:305-311
Mandels, M., Andreotti, R., Roche, C. 1976. Measurment of Saccharifying Cellulase. Journal of Biotechnol. and Bioeng. Symp. 6:21-33
Miller, G. L. 1959. Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar. Journal of Anal. Chem 31 : 51-56
Mingardon, F., Bagert, J.D., Maisonnier, C., Trudeau, D.L., dan Arnold, F.H. 2011. Comparison of Family 9 Cellulases from Mesophilic and Thermophilic Bacteria Comparison of Family 9 Cellulases from Mesophilic and Thermophilic Bacteria. Journal of Applied and Environmental Microbiology 77: 1436 1442
Murao, S., Sakamoto, R., Arai, M. 1988. Cellulase of Aspergillus aculeatus : Methods in Enzymology. In: Wood, W.A. dan Kellog, S.T. London : Academic Press Inc
Nochure, S.V., Roberts, M.F., Demai, A.I. 1993. True Cellulase Production by Clostridium thermocellum Grown on Different Carbon Sources. Journal of Biotech Letters 15:641-646
Oikawa, T., Tsukagawa, Y., Soda, K. 1998. Endo-ß-Glucanase Secreted By A Psychrotrophic Yeast: Purification And Characterization. Journal of Biotechnol. Biochem 62 : 1751-1756
Pelczar, J., Michael dan Chan E.C.S. 1986. Dasar-dasar Mikrobiologi. Diterjemahkan oleh Hadioetomo, R. S., Jakarta: Universitas Indonesia Press
Rabelo, M.C., Fontes, C.M.L., Rodrigues, S. 2011. Stability Study of Crude Dextransuscrase from Leuconostoc citreum NRRI B-742. Indiana Journal of Microbiology 51:11-170
Rahman, R.N., Geok, L.P., Basri, M., Saleh, A.B. 2004. Physical Factors Affecting the Production of Organic Solvent-Tolerant Protease by Pseudomonas aeruginosa Strain K. Journal of Bioresource Technology 96:429-43
Rai, P., Tiwari, S., dan Gaur, R. 2012. Optimization Of Process Parameters For Cellulase Production By Novel Thermotolerant Yeast. Journal of BioResources 7 : 5401-5414
Ray, M.K., Devi, K.U., Kumar, G., Dhivaji, S. 1992. Extracellular Protease from the Antartic Yeast Candida humicola. Journal of Applied and Enviromental Microbiology 58:1918-1923
Romsaiyud, A., Songkasiri, W., Nopharatana, A., dan Chaiprasert, P. 2009. Combination Effect of pH and Acetate on Enzymatic Cellulose Hydrolysis. Journal of Environmental Sciences 21:965 970
Sharada, R., Venkateswarlu, G., Venkateswar, S., Dan Anandra. A:lications Of Cellulases – Review. International Journal Of Pharmaceutical 4 : 424-437
Singh, A., Singh, N., Bishnoi, N. R. 2009. Production of Cellulases by Aspergillus heteromorphus from Wheat Straw Under Submerged Fermentation. Internasional Journal of Sci. Environ. Eng. 1 : 23-26
Suhartono, M.T. 1989. Enzim dan Bioteknologi. Bogor:PAU Bioteknologi IPB
Taha, A. S. J., Taha, A. J., Faisal, Z. G. 2015. Purification and Kinetic Study on Cellulase Produced by Local Trichoderma viride. Journal of Nature and Science 13:87-90
Vortruba, J., Pazlarova, J., Dvorakova, M., Vachora, L., Strnadova, M., Kucerova, H., Vinter, V., Zourabian, R., Chaloupka, J. 1991. External Factors Involved in the Regulation of Synthesis of an Extracellular Proteinase in Bacillus megaterium: Effect of Temperature. Journal of Aplied Microbiology and Biotechnology 35:352-357
Wilson, D. B. 2009. Cellulases and Biofuels. Journal of Energy Biotechnology 20 : 295-299
Zhang L, Chi ZM. 2007. Screening And Identification Of A Cellulase Producing Marine Yeast And Optimization Of Medium And Cultivation Conditions For Cellulase Production. Journal of Ocean Univer China 37 : 101–108
Zhang, Y., Zhu, Y., Zhu, Y. and Li, Y. 2009. The Importance of Engineering Physiological Functionality Into Microbes. Journal of Biotechnol 27 : 664-672
Agarwal, T., Saxena, M.K., Chandrawat, M.P.S. 2014. Production and Optimization of Cellulase Enzyme By Pseudomonas aeruginosa MTCC 4643 Using Sawdust as A Substrate. International Journal of Scientific and Research Publications. 4:1-3
Agbogbo, F.K., Hagensen, F.D., Milam, D., dan Wenger, K. S. 2008. Fermentation Of Acid-Pretreated Corn Stover To Ethanol Without Detoxification Using Pichia stipitis. Journal of Application Biochemical Biotechnology 145:53–58
Alami, N.H., dan Shovitri, M. 2015. Studi Lanjut Marine Yeast sebagai Biofertilizer Komersial. Laporan Akhir Penelitian Pemula. Surabaya : Jurusan Biologi Fakultas Matematika dan Ilmu Pengetahuan Alam Institut Teknologi Sepuluh Nopember
Apun, K. 1995. Cellulase Production. National Centre for Biotechnology Education. Malaysia
Asaduzzaman. 2007. Standardization of Yeast Groth Curve from Several Curves with Different Initial Sizes. Tesis. Gȍteborg. Departement of Mathematical Statistics, Chalmers University of Technology and Gȍteborg University
Ates, S., Elibol, M., Mavituna, F. 1997. Production of Actinorhodin by Streptomyces coelicolor in Batch and Fed Batch Cultures. Journal of Process Biochemistry 32:273-278
Ballesteros, M., Olivia, J.M., Negro, M.J., Manzanares, P., Ballesteros, I. 2004. Ethanol from Lignocellulosic Materials by a Simultaneous Saccharification and Fermentation Process (SFS) with Kluyveromyces marxianus CECT 10875. Journal of Process Biochemichal 39:1843-1848
Chasanah, E., Dini, I.R., Mubarik, N.R. 2013. Karakterisasi Enzim Selulase PMP 1026Y dari Limbah Pengolahan Agar. Jurnal JPB Perikanan 8:103-114
Chi, Z., Zhang, T., Liu, G., Li, J., Wang, X. 2009. Production, Characterization And Gene Cloning Of The Extracellular Enzymes From The Marine-Derived Yeasts And Their Potential A:lications. Journal of Biotechnology Adv 27:236–255
Choder M. A. 1991. General Topoisomerase I-Dependent Transcriptional Repression In The Stationary Phase In Yeast. Genes Dev. 5:2315–2326.
Das, A., Paul, T., Halder, S.K., Maity, C., Mohapatra. P. K. D., Pati, B. R., Mondal, K.C. 2013. Study on Regulation of Growth and Biosynthesis of Cellulolytic Enzymes from Newly Isolated Aspergillus fumigatus ABK9. Journal of Microbiology 62:31-43
Englard, S., dan Sifters, S. 1990. Precipitation Techniques. Journal of Methods Enzymology 182:285-300
Fardiaz, S. 1992. Mikrobiologi Pangan I. Jakarta:Gramedia Pustaka Utama.
Ferreira, S.M.P., Duarte, A.P., Queiroz, J.A., dan Domingues, F.C. 2009. Influence of Buffer Systems on Trichoderma reesei Rut C-30 Morphology and Cellulase Production. Electronic Journal of Biotechnology 12:3
Goldschmidt, F. 2008. From Cellulose to Ethanol: Engineering Microorganisms to Produce Biofuel. Department Environmental Sciences. Institute of Biogeochemistry and Pollutant Dynamics. Zurich
Golias, H., Dumsday, G.J., Stanley, G.A., dan Pamment, N.B. 2002. Evaluation of a Recombinat Klebsiella oxytoca strain for Ethanol Production from Ethanol Production from Cellulose by Simultaneus Saccharification and Fermentation: Comparison with Native Cellobiose Utilising Yeast Strains and Performance in Co-Culture with Thermotolerant Yeast and Zymomonas mobilis. Journal of Biotechnology 96:155-168
Harshvardhan, K.., Mishr, A., Jha, B. 2013. Purification and Characterization of Cellulase from A Marine Bacillus sp. H1666: A Potential Agent For Single Step Saccharification Of Seaweed Biomass. Journal of Molecular Catalysis B: Enzymatic 93:51-56
Ijaz, A., Anwar, Z., Zafar, Y., Hussain, I., Muhammad, A., Irshad, M., Mehmood, S. 2011. Optimization of Cellulase Enzyme Production from Corn Cobs Using Alternia alternata by Solid State Fermentation. Journal of Cell and Molecular Biology 9 : 51-56
Ire, F. S., Okolo, B. N., Moneke, A. N., Odibo, F. J. C. 2011. Influence of Cultivation Conditions on The Production of A Protease From Aspergillus carbonarius Using Submerged Fermentation. African Journal of Food Science 5:353-365
Johri, B.N., Alluralde, J.D., Klein, J. 1990. Lipase Production by Free and Immobilized Protoplasts Sporotrichum thermophile. Journal of Applied Microbiology and Biotechnology 33:367-371
Kadam, K.L., Schmidt, S.L. 1997. Evaluation of Candida acidothermophilus in Ethanol Production from Lignocellulosic Biomass. Journal of A.l.Microbiol. Biotechnol 48 : 709-713.
Kathiresan, K., Saravanakumar, K., Senthilraja, P. 2011. Bioethanol Production by Marine Yeast Isolated from Coastal Mangrove Sediment. Journal of Int. Multidiscip Res 1 : 19-24
Kirk, O., Borchert, T. V., and Fuglsang, C. C. 2002. Industrial enzyme a:lications. Journal of Curr. Opin. Biotechnol 13 : 345-351
Kurtzman, C.P. dan Fell, J.W. 1998. The Yeasts a Taxonomic Study, 4th edn. Amsterdam:Elsevier
Lehninger, A.L. 2000. Dasar-Dasar Biokimia. Jakarta: Erlangga
Lu, W., Li, D., Wu, Y. 2003, Influence of Water Activity and Temperature on Xylanase Biosynthesis in Pilotscale Solidstate Fermentation by Aspergillus sulphurous. Journal of Enzyme Microbiol. Technology 32:305-311
Mandels, M., Andreotti, R., Roche, C. 1976. Measurment of Saccharifying Cellulase. Journal of Biotechnol. and Bioeng. Symp. 6:21-33
Miller, G. L. 1959. Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar. Journal of Anal. Chem 31 : 51-56
Mingardon, F., Bagert, J.D., Maisonnier, C., Trudeau, D.L., dan Arnold, F.H. 2011. Comparison of Family 9 Cellulases from Mesophilic and Thermophilic Bacteria Comparison of Family 9 Cellulases from Mesophilic and Thermophilic Bacteria. Journal of Applied and Environmental Microbiology 77: 1436 1442
Murao, S., Sakamoto, R., Arai, M. 1988. Cellulase of Aspergillus aculeatus : Methods in Enzymology. In: Wood, W.A. dan Kellog, S.T. London : Academic Press Inc
Nochure, S.V., Roberts, M.F., Demai, A.I. 1993. True Cellulase Production by Clostridium thermocellum Grown on Different Carbon Sources. Journal of Biotech Letters 15:641-646
Oikawa, T., Tsukagawa, Y., Soda, K. 1998. Endo-ß-Glucanase Secreted By A Psychrotrophic Yeast: Purification And Characterization. Journal of Biotechnol. Biochem 62 : 1751-1756
Pelczar, J., Michael dan Chan E.C.S. 1986. Dasar-dasar Mikrobiologi. Diterjemahkan oleh Hadioetomo, R. S., Jakarta: Universitas Indonesia Press
Rabelo, M.C., Fontes, C.M.L., Rodrigues, S. 2011. Stability Study of Crude Dextransuscrase from Leuconostoc citreum NRRI B-742. Indiana Journal of Microbiology 51:11-170
Rahman, R.N., Geok, L.P., Basri, M., Saleh, A.B. 2004. Physical Factors Affecting the Production of Organic Solvent-Tolerant Protease by Pseudomonas aeruginosa Strain K. Journal of Bioresource Technology 96:429-43
Rai, P., Tiwari, S., dan Gaur, R. 2012. Optimization Of Process Parameters For Cellulase Production By Novel Thermotolerant Yeast. Journal of BioResources 7 : 5401-5414
Ray, M.K., Devi, K.U., Kumar, G., Dhivaji, S. 1992. Extracellular Protease from the Antartic Yeast Candida humicola. Journal of Applied and Enviromental Microbiology 58:1918-1923
Romsaiyud, A., Songkasiri, W., Nopharatana, A., dan Chaiprasert, P. 2009. Combination Effect of pH and Acetate on Enzymatic Cellulose Hydrolysis. Journal of Environmental Sciences 21:965 970
Sharada, R., Venkateswarlu, G., Venkateswar, S., Dan Anandra. A:lications Of Cellulases – Review. International Journal Of Pharmaceutical 4 : 424-437
Singh, A., Singh, N., Bishnoi, N. R. 2009. Production of Cellulases by Aspergillus heteromorphus from Wheat Straw Under Submerged Fermentation. Internasional Journal of Sci. Environ. Eng. 1 : 23-26
Suhartono, M.T. 1989. Enzim dan Bioteknologi. Bogor:PAU Bioteknologi IPB
Taha, A. S. J., Taha, A. J., Faisal, Z. G. 2015. Purification and Kinetic Study on Cellulase Produced by Local Trichoderma viride. Journal of Nature and Science 13:87-90
Vortruba, J., Pazlarova, J., Dvorakova, M., Vachora, L., Strnadova, M., Kucerova, H., Vinter, V., Zourabian, R., Chaloupka, J. 1991. External Factors Involved in the Regulation of Synthesis of an Extracellular Proteinase in Bacillus megaterium: Effect of Temperature. Journal of Aplied Microbiology and Biotechnology 35:352-357
Wilson, D. B. 2009. Cellulases and Biofuels. Journal of Energy Biotechnology 20 : 295-299
Zhang L, Chi ZM. 2007. Screening And Identification Of A Cellulase Producing Marine Yeast And Optimization Of Medium And Cultivation Conditions For Cellulase Production. Journal of Ocean Univer China 37 : 101–108
Zhang, Y., Zhu, Y., Zhu, Y. and Li, Y. 2009. The Importance of Engineering Physiological Functionality Into Microbes. Journal of Biotechnol 27 : 664-672