Effect of boiling temperature and time on total flavonoids, total phenols, and radical scavenging activity of decoction water fresh gotu kola (Centella asiatica (L.) Urb.)
Main Article Content
Abstract
Gotu kola (Centella asiatica (L.) Urb.) possesses significant pharmacological effects as a versatile herb, particularly in wound healing, neuroprotection, and cardiovascular health. Some people derive the benefits of medicinal plants by boiling and consuming them. The boiling process significantly impacts the biochemical composition, including total phenols, total flavonoids, and antioxidant activity. This study aims to investigate the impact of temperature and time on total flavonoids, total phenols, and radical scavenging activity during the boiling process of fresh C. asiatica. Three different temperature treatments (75°C, 85°C, 95°C) and four different boiling time treatments (5, 10, 15, and 20 minutes) were used to assess the total flavonoid content, total phenolics, and radical scavenging activity using the DPPH method. Quantitative data analysis employed DMRT and PCA techniques to ascertain the impact and correlation among factors. The results showed that temperature and boiling time significantly influenced total flavonoids, total phenols, and radical scavenging activity. Maximum levels of total flavonoids, total phenols, and radical scavenging activity were achieved in C. asiatica boiling water after boiling at 95 °C for 20 minutes. Temperature, total flavonoids, and total phenols have a strong correlation, where total flavonoids and total phenols increase as the boiling temperature increases.
Article Details
References
[2] Tan S C, Bhattamisra S K, Chellappan D K and Candasamy M 2021 Appl. Sci. 11
[3] KK A 2020 Int. J. Adv. Acad. Stud. 2 369–72
[4] Gray N E, Alcazar Magana A, Lak P, Wright K M, Quinn J, Stevens J F, Maier C S and Soumyanath A 2018 Rev. 17 161–94
[5] Pittella F, Dutra R C, Junior D D, Lopes M T P and Barbosa N R 2009 Int. J. Mol. Sci. 10 3713–21
[6] Zhao Y, Shu P, Zhang Y, Lin L, Zhou H, Xu Z, Suo D, Xie A and Jin X 2014 Oxid. Med. Cell. Longev. 2014 1–7
[7] Gnanapragasam A, Kumar Ebenezar K, Sathish V, Govindaraju P and Devaki T 2004 Life Sci. 76 585–97
[8] Wahyuningsih S, Syukur A and Khairuddin K 2022 J. Biol. Trop. 22 1057–70
[9] Oktavi Azzahra E, Agustina A and Masyithoh G 2024 IOP Conference Series: Earth and Environmental Science vol 1362 (Institute of Physics)
[10] Sari R P, Yusro F and Mariani Y 2021 J. Biol. Trop. 21 324–35
[11] Reddy S 2023 Support pp 347–68
[12] Dean M 2024 Golden Ratio Data Summ. 4 6–18
[13] de Lima Marsiglia W I M, Oliveira L de S C, Lucas Jacinto Almeida R, Santos N C, da Silva Neto J M, Santiago  M, de Melo B C A and Honorato da Silva F L 2023 J. Indian Chem. Soc. 100 100995
[14] Fauziah I N, Prangdimurti E and Palupi N S 2023 IOP Conf. Ser. Earth Environ. Sci. 1200 12014
[15] Pattarathitiwat P, Chinvongamorn C and Sansenya S 2021 Prev. Nutr. Food Sci. 26 92–9
[16]Salamatullah A M, Özcan M M, Alkaltham M S, Uslu N and Hayat K 2021 J. Food Process. Preserv. 45
[17] Irondi E A, Akintunde J K, Agboola S O, Boligon A A and Athayde M L 2017 Food Sci. Nutr. 5 233–42
[18] Sawicki T, Wiczkowski W, Hrynkiewicz M, Bączek N, Hornowski A, Honke J and Topolska J 2021 Int. J. Food Sci. Technol. 56 629–38
[19] Sun H, Mu T, Xi L and Song Z 2014 Agric. Food Chem. 62 8982–9
[20] Sharma K, Ko E Y, Assefa A D, Ha S, Nile S H, Lee E T and Park S W 2015 J. Food Drug Anal. 23 243–52
[21] EYMİR A, KARAKAVUK E, EROĞLU Z and BENZER F 2023 Int. J. Agric. Environ. Food Sci. 7 253–9
[22] Timón M L, Andrés A I and Petrón M J 2024 Foods 13 1802
[23] Pereira V P, Knor F J, Vellosa J C R and Beltrame F L 2014 Rev. Bras. Plantas Med. 16 490–8
[24] Esmaeili A K, Taha R M, Mohajer S and Banisalam B 2015 Biomed Res. Int. 2015 1–11
[25] Cianciosi D, Forbes‐Hernández T Y, Giampieri F, Zhang J, Ansary J, Pacetti M, Quiles J L, Simal‐Gandara J and Battino M 2020 eFood 1 85–93
[26] Kędzierska-Matysek M, Stryjecka M, Teter A, Skałecki P, Domaradzki P and Florek M 2021 Molecules 26 1810
[27] Ulewicz-Magulska B and Wesolowski M 2023 Antioxidants 12 2039
[28] Zielinski A A F, Ávila S, Ito V, Nogueira A, Wosiacki G and Haminiuk C W I 2014 J. Food Sci. 79
[29] Widodo H, Sismindari, Asmara W and Rohman A 2020 Food Res. 4 127–34
[30] Widodo H 2021 Aktivitas Antioksidan Tumbuhan Terpilih Dari Ramuan Empiris Untuk Penyakit Liver: Kajian Fisika-Kimia dan Biologi Molekular (Gadjah Mada University, Yogyakarta)
[31] Puspitasari A D and Prayogo L S 2016 J. Inov. Tek. Kim. 1 104–8
[32] Ramalho S A, Nigam N, Oliveira G B, de Oliveira P A, Silva T O M, dos Santos A G P and Narain N 2013 Food Res. Int. 51 155–61
[33] Fitriansyah S N, Riasari H, Melati N and Simanjuntak N 2024 Pharma Xplore J. Sains dan Ilmu Farm. 9 14–22
[34] Alide T, Wangila P and Kiprop A 2020 BMC Res. Notes 13 1–7
[35] GA S and MS M 2016 Nat. Prod. Chem. Res. 04